Plasmid

Part:BBa_K5048042:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid ccdBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5817
    Illegal BamHI site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 990
    Illegal NgoMIV site found at 4037
    Illegal NgoMIV site found at 4197
    Illegal NgoMIV site found at 5785
    Illegal AgeI site found at 417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 245
    Illegal SapI site found at 399
    Illegal SapI.rc site found at 3116


Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.



Source

complete synthesis

References